The Spirit-NOPA is a family of automated non-collinear optical parametric amplifiers (NOPA) specifically built and optimized for the industry-proven Spirit® HE and Spirit® One™ ultrafast lasers. The turn-key, high repetition rate Spirit femtosecond laser combines with the Spirit-NOPA to create a powerful, user-friendly tunable, high repetition rate source of ultrashort pulses for in-vivo, deep tissue imaging in neuroscience, spectroscopy, and other ultrafast scientific applications.